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pRS406 ATG1-ATG17-3'UTR
(Plasmid #166848)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 166848 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS406
  • Total vector size (bp) 9024
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATG1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    4893
  • Entrez Gene
    ATG1 (a.k.a. YGL180W, APG1, AUT3, CVT10)
  • Promoter ATG1 Promoter

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Linearize with StuI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS406 ATG1-ATG17-3'UTR was a gift from Daniel Klionsky (Addgene plasmid # 166848 ; http://n2t.net/addgene:166848 ; RRID:Addgene_166848)
  • For your References section:

    The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy. Gatica D, Hu G, Liu X, Zhang N, Williamson PR, Klionsky DJ. Mol Cell. 2019 Jan 17;73(2):314-324.e4. doi: 10.1016/j.molcel.2018.11.002. Epub 2018 Dec 6. 10.1016/j.molcel.2018.11.002 PubMed 30527663