GFP-p58
(Plasmid #166942)

• Purpose: mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with GFP
• Depositing Lab: Jennifer Lippincott-Schwartz
• Publication: Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70. doi: 10.1083/jcb.200107045. Epub 2001 Nov 12.

Backbone

• Vector backbone: GFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p58
• Alt name: ERGIC-53
• Alt name: LMAN1
• Species: R. norvegicus (rat)
• Entrez Gene: Lman1 (a.k.a. p58)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GFP (N terminal on insert)
  • chicken lysozyme signal peptide (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there are two mutations in p58-L405Q, R419S Depositor confirms these do not affect plasmid function.