pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt
(Plasmid
#166995)
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PurposeTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166995 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTermination module:PolyU(0), Hairpin = 0 nt
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0nt was a gift from Julius Lucks (Addgene plasmid # 166995 ; http://n2t.net/addgene:166995 ; RRID:Addgene_166995) -
For your References section:
RNA Sequence and Structure Determinants of Pol III Transcriptional Termination in Human Cells. Verosloff MS, Corcoran WK, Dolberg TB, Bushhouse DZ, Leonard JN, Lucks JB. J Mol Biol. 2021 Jun 25;433(13):166978. doi: 10.1016/j.jmb.2021.166978. Epub 2021 Apr 1. 10.1016/j.jmb.2021.166978 PubMed 33811918
