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RNA Sequence and Structure Determinants of Pol III Transcriptional Termination in Human Cells.
Verosloff MS, Corcoran WK, Dolberg TB, Bushhouse DZ, Leonard JN, Lucks JB
J Mol Biol. 2021 Jun 25;433(13):166978. doi: 10.1016/j.jmb.2021.166978. Epub 2021 Apr 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
159479 pAV-U6+27-Linear-1_PolyU(1)Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159480 pAV-U6+27-Linear-1_PolyU(2)Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159481 pAV-U6+27-Linear-1_PolyU(3)Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159482pAV-U6+27-Linear-1_PolyU(4)_Tornado-CornTests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159483 pAV-U6+27-Linear-1_PolyU(5)_Tornado-CornTests for the impact of 5 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159484 pAV-U6+27-Linear-1_PolyU(6)_Tornado-CornTests for the impact of 6 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159485 pAV-U6+27-Linear-1_PolyU(7)_Tornado-CornTests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159486 pAV-U6+27-Linear-1_PolyU(8)_Tornado-CornTests for the impact of 8 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159487 pAV-U6+27-Linear-2_PolyU(1)_Tornado-CornTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159488 pAV-U6+27-Linear-2_PolyU(2)_Tornado-CornTests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159490 pAV-U6+27-Linear-2_PolyU(4)_Tornado-CornTests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159493 pAV-U6+27-Linear-2_PolyU(7)_Tornado-CornTests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159495 pAV-U6+27-Linear-1_PolyU(1)_HairpinDistance(0)nts_Tornado-CornTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
159496 pAV-U6+27-Linear-1_PolyU(4) _HairpinDistance(0)_Tornado-CornTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
159498 pAV-U6+27-Linear-1_PolyU(4)_HairpinDistance(10)nts_Tornado-CornTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
159499 pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(0)nts_Tornado-CornTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
159501 pAV-U6+27-Linear-2_PolyU(1)_HairpinDistance(10)nts_Tornado-CornTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
159502 pAV-U6+27-Linear-2_PolyU(4)_HairpinDistance(10)nts_Tornado-CornTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
159503 pAV-U6+27-Linear-3_PolyU(1)_Tornado-CornTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.
159504 pAV-U6+27-Linear-3_PolyU(1)_HairpinDistance(10)nts_Tornado-CornTests for the impacts 1 uracil in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
159506 pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(10)nts_Tornado-CornTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 10 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
159508 pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(0)nts_Tornado-CornTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with an immediately upstream hairpin have on human polymerase III transcription elongation from a U6 promoter.
159511 pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(16)nts_Tornado-CornTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 16 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
159514 pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(19)nts_Tornado-CornTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 19 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
159515 pAV-U6+27-Linear-3_PolyU(4)_HairpinDistance(20)nts_Tornado-CornTests for the impacts 4 uracils in a poly-uracil tract in conjunctiuon with a hairpin 20 nts upstream have on human polymerase III transcription elongation from a U6 promoter.
166984GLN, Linear-3, PolyU(1)Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
166985GLN, Linear-3, PolyU(4)Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
166986GLN, Linear-3, PolyU(1), Hairpin Distance = 0ntTests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
166987GLN, Linear-3, PolyU(4), Hairpin Distance = 0ntTests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.
166988GLY, Linear-3, PolyU(1)Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.
166989GLY, Linear-3, PolyU(4)Tests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
166990GLY, Linear-3, PolyU(1), Hairpin Distance = 0ntTests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
166991GLY, Linear-3, PolyU(4), Hairpin Distance = 0ntTests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.
166992pAV-U6+27-Linear-1, PolyU(0)Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.
166993pAV-U6+27Linear-1, PolyU(0), Hairpin Distance = 0ntTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.
166994pAV-U6+27Linear-2, PolyU(0)Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.
166995pAV-U6+27Linear-2, PolyU(0), Hairpin Distance = 0ntTests for the impact of a synthetic linear tract in conjunction with a hairpin motif on human polymerase III transcription from a U6 promoter.

Antibodies from Article