pLenti-3xFLAG-Puro PAK5 K478R/479R S364L
(Plasmid #167199)

• Purpose: puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant
• Depositing Lab: Christin Burd
• Publication: LaPak et al Oncotarget. 2018 May 22;9(39):25386-25401. doi: 10.18632/oncotarget.25356. eCollection 2018 May 22.

Backbone

• Vector backbone: p-Lenti-puro
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PAK5 K478R/479R S364L
• Alt name: PAK7 K478R/479R S364L
• Species: H. sapiens (human)
• Promoter: UBC

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTAAATTGTCCGCTAAATTCTGGCCGTT
• 3′ sequencing primer: CACACCGGCCTTATTCCAAGCG

Resource Information

• Supplemental Documents:
  • #879 pLenti-3xFLAG PAK7 K478-9R S364L.gb
• A portion of this plasmid was derived from a plasmid made by: pDEST-PAK5 was kindly provided by Dr. J. Brognard

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry