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Melanoma-associated mutants within the serine-rich domain of PAK5 direct kinase activity to mitogenic pathways.
LaPak KM, Vroom DC, Garg AA, Guan X, Hays JL, Song JW, Burd CE
Oncotarget. 2018 May 22;9(39):25386-25401. doi: 10.18632/oncotarget.25356. eCollection 2018 May 22. PubMed Article

Plasmids from Article

ID Plasmid Purpose
167168pLenti--Puro-3xFLAG PAK5 S364Lpuromycin resistant lentiviral vector for expression of PAK5 S364L
167179pLenti-3xFLAG-Puro PAK5 V593Ipuromycin resistant lentiviral vector for expression of PAK5 V593I
167180pLenti-3xFLAG-Puro PAK5 K478R K469Rpuromycin resistant lentiviral vector for expression of PAK5 K478R K469R
167181pLenti-3xFLAG-Puro PAK5 T538Npuromycin resistant lentiviral vector for expression of PAK5 T538N
167192pLenti-3xFLAG-Puro PAK5 M173Ipuromycin resistant lentiviral vector for expression of PAK5 M173I
167193pLenti-3xFLAG-Puro PAK5 E144Kpuromycin resistant lentiviral vector for expression of PAK5 E144K
167195pLenti-3xFLAG-Puro PAK5 S573Npuromycin resistant lentiviral vector for expression of PAK5 S573N
167196pLenti-3xFLAG-Puro PAK5 K478R/479R E144Kpuromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant
167197pLenti-3xFLAG-Puro PAK5 K478R/479R M173Ipuromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant
167198pLenti-3xFLAG-Puro PAK5 K478R/479R E294Kpuromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant
167199pLenti-3xFLAG-Puro PAK5 K478R/479R S364Lpuromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant
167200pLenti-3xFLAG-Puro PAK5 K478R/479R D421Npuromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

Antibodies from Article