XE120 CAMKII-K42M 1-271-CS2+
(Plasmid #16734)

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Depositing Lab

Randall Moon
Publication

Xenopus Expression Plasmids (unpublished) ( How to cite )
Sequence Information
- Sequences (1)

Full plasmid sequence is not available for this item.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	16734	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pCS2+
(Search Vector Database)
Backbone size w/o insert (bp) 4095
Vector type
Xenopus Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
CAMKII-K42M 1-271
Alt name
CAMKII
Alt name
caM kinase
Species
R. norvegicus (rat)
Mutation
C-terminal truncation of K42M kinase
Entrez Gene
Camk2a (a.k.a. PK2CDD, PKCCD)

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site EcoR1 (unknown if destroyed)
3′ cloning site EcoR1 (unknown if destroyed)
5′ sequencing primer SP6

(Common Sequencing Primers)

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.

Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

XE120 CAMKII-K42M 1-271-CS2+ was a gift from Randall Moon (Addgene plasmid # 16734 ; http://n2t.net/addgene:16734 ; RRID:Addgene_16734)

XE120 CAMKII-K42M 1-271-CS2+ (Plasmid #16734)