|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16896||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Entrez Geneprickle2b (a.k.a. cb886, im:7138215, pk2, prickle2)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Partial length prickle-2, contains the entire protein except the last 75 amino acids (predicted). Cloned into the BamH1 and Cla1 sites of CS2P+.
To make antisense probe: cut with BamH1, transcribe with T7. For the sense strand cut with Cla1 and transcribe with SP6.
Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP46 Prickle-2 was a gift from Randall Moon (Addgene plasmid # 16896 ; http://n2t.net/addgene:16896 ; RRID:Addgene_16896)