pCAG-Beatrix
(Plasmid #167528)

• Purpose: Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.
• Depositing Lab: Gian Michele Ratto
• Publication: Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi: 10.1038/s41467-020-19864-w.

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 4775
• Total vector size (bp): 7779
• Vector type: Mammalian Expression, Mouse Targeting, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Beatrix
• Alt name: DsRed2, GFP-P2A-Cre
• Species: G. gallus (chicken), O. cuniculus (rabbit); Bacteriophage P1, Aequorea victoria, Discosoma sp., Oryctolagus cuniculus
• Insert Size (bp): 2996
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: Not (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• Supplemental Documents:
  • pCAG-Beatrix.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry