pPM2212
(Plasmid
#167552)
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PurposeEncodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHC79
- Backbone size w/o insert (bp) 6000
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Vector typeCosmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namerfb region of Shigella flexneri serotype 2a
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Alt nameCosmid clone
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SpeciesShigella flexneri
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPM2212 was a gift from Renato Morona (Addgene plasmid # 167552 ; http://n2t.net/addgene:167552 ; RRID:Addgene_167552) -
For your References section:
Genetic analysis of the rfb region of Shigella flexneri encoding the Y serotype O-antigen specificity. Macpherson DF, Morona R, Beger DW, Cheah KC, Manning PA. Mol Microbiol. 1991 Jun;5(6):1491-9. doi: 10.1111/j.1365-2958.1991.tb00795.x. 10.1111/j.1365-2958.1991.tb00795.x PubMed 1724058
