pMF19
(Plasmid
#113662)
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PurposeContaining the wbbL (rhamnosyltransferase) gene cloned for mutant complementation
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEXT21
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namewbbL
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Alt namerhamnosyltransferase
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Insert Size (bp)-4
- Promoter Ptac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer TGCTCGCGCCCTGGAATG
- 3′ sequencing primer GAACATTGAAATGGTAT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please Note: The plasmid contains two wbbL inserts, which is not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMF19 was a gift from Miguel Valvano (Addgene plasmid # 113662 ; http://n2t.net/addgene:113662 ; RRID:Addgene_113662) -
For your References section:
The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat. Feldman MF, Marolda CL, Monteiro MA, Perry MB, Parodi AJ, Valvano MA. J Biol Chem. 1999 Dec 3;274(49):35129-38. PubMed 10574995
