XE161 XDshHA-triple pt mutant-CS2+
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16779||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeXenopus Expression
Growth in Bacteria
SpeciesX. laevis (frog)
Mutationtriple point mutation outside of the PDZ binding domain
Entrez Genedvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
HA tagged Xenopus Dishevelled with a triple point mutation outside of the PDZ binding domain, that does not block binding to Dapper.
Notes for Use: Linearize with either Not1 or Asp718
Reference: B. Cheyette et al. (2002). Developmental Cell 2: 449-461
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE161 XDshHA-triple pt mutant-CS2+ was a gift from Randall Moon (Addgene plasmid # 16779 ; http://n2t.net/addgene:16779 ; RRID:Addgene_16779)