|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15491||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesX. laevis (frog)
Insert Size (bp)3100
MutationDeletion of PDZ domain
Entrez Genedvl2.L (a.k.a. XELAEV_18019661mg, dishevelled, dsh, dvl, dvl2, xdd1, xdsh)
/ Fusion Protein
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Xdd1 is a mutant Xdsh with a 200 bp internal deletion of the conserved PDZ/DHR domain. See author's map for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Myc-Xdd1-pCS2 was a gift from Sergei Sokol (Addgene plasmid # 15491 ; http://n2t.net/addgene:15491 ; RRID:Addgene_15491)
For your References section:Analysis of Dishevelled signalling pathways during Xenopus development. Sokol SY. Curr Biol. 1996 Nov 1. 6(11):1456-67. 10.1016/S0960-9822(96)00750-6 PubMed 8939601
Map uploaded by the depositor.