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NAX_CAGGS_CAS9_m4_VPR_EBFP2
(Plasmid #167881)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167881 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 167881-D50 50 μg of DNA in Tris buffer $495
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DNA 167881-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pB-CAGGS
  • Vector type
    CRISPR
  • Selectable markers
    EBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-VPR
  • Species
    Synthetic
  • Mutation
    D10A, D839A, H840A, N863A

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NAX_CAGGS_CAS9_m4_VPR_EBFP2 was a gift from Alejandro Chavez & Nan Cher Yeo (Addgene plasmid # 167881 ; http://n2t.net/addgene:167881 ; RRID:Addgene_167881)

  • For your References section:

    Generation and application of a versatile CRISPR toolkit for mammalian cell engineering. Nghi Dang , Alissa Lance-Byrne, Angela Tung, Xiaoge Guo, Ryan J Cecchi, Joanna Buchthal, Alejandro Chavez, Nan Cher Yeo. Synthetic Biology, Volume 6, Issue 1, 2021 10.1093/synbio/ysab033
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