XE130 beta2AR Fz2-Cs2+
(Plasmid #16794)

• Depositing Lab: Randall Moon
• Publication: Xenopus Expression Plasmids (unpublished)

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: beta 2 AR Fz2
• Alt name: beta 2 adrenergic receptor
• Alt name: Adrb2
• Alt name: Frizzled
• Species: R. norvegicus (rat)
• Entrez Gene: Fzd1
• Entrez Gene: Adrb2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Chimera of beta 2 adrenergic receptor (extracellular and.
transmembrane domains) and rat frizzled-2 (intracellular). Activated by isoproterenol and inhibited by propranolol.

Ref: X. Lui et al., PNAS, 1999, 96:14383-14388 and M. Kuehl et al.,
2000, JBC 275:12701-12711.