XE182 rat Frizzled-1-HA-pCD
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16818||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typeXenopus Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)2044
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The original rat Frizzled-1 cDNA, tagged with an HA epitope at the Sty1 site of Frizzled1 (third extracellular loop), was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The untagged part of the insert was published by Wang et al., JBC 271: 4468 (1996). The HA-tag insert was made by Jeff Brown, and the remainder of this unpublished construct was generated by Julia Yang-Snyder.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE182 rat Frizzled-1-HA-pCD was a gift from Randall Moon (Addgene plasmid # 16818 ; http://n2t.net/addgene:16818 ; RRID:Addgene_16818)