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pSEVA221rAPOBEC1-T7RNAP
(Plasmid #167979)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 167979 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSEVA221
  • Backbone manufacturer
    Victor de Lorenzo
  • Backbone size w/o insert (bp) 3719
  • Total vector size (bp) 7918
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    rAPOBEC1-T7RNAP
  • Species
    Synthetic
  • Insert Size (bp)
    4199
  • Entrez Gene
    T7p07 (a.k.a. T7p07)
  • Promoter TetR-Ptet
  • Tags / Fusion Proteins
    • T7 tag (N terminal on insert)
    • T7RNAP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVA221rAPOBEC1-T7RNAP was a gift from Luis Ángel Fernández (Addgene plasmid # 167979 ; http://n2t.net/addgene:167979 ; RRID:Addgene_167979)
  • For your References section:

    In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9. Alvarez B, Mencia M, de Lorenzo V, Fernandez LA. Nat Commun. 2020 Dec 22;11(1):6436. doi: 10.1038/s41467-020-20230-z. 10.1038/s41467-020-20230-z PubMed 33353963