pSEVA221rAPOBEC1-T7RNAP
(Plasmid #167979)

• Purpose: Expresses fusion protein rAPOBEC1-T7RNAP in E. coli
• Depositing Lab: Luis Ángel Fernández
• Publication: Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi: 10.1038/s41467-020-20230-z.

Backbone

• Vector backbone: pSEVA221
• Backbone manufacturer: Victor de Lorenzo
• Backbone size w/o insert (bp): 3719
• Total vector size (bp): 7918
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: rAPOBEC1-T7RNAP
• Species: Synthetic
• Insert Size (bp): 4199
• Entrez Gene: T7p07 (a.k.a. T7p07)
• Promoter: TetR-Ptet
• Tags / Fusion Proteins:
  • T7 tag (N terminal on insert)
  • T7RNAP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pSEVA221rAPOBEC1-T7RNAP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry