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In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9.
Alvarez B, Mencia M, de Lorenzo V, Fernandez LA
Nat Commun. 2020 Dec 22;11(1):6436. doi: 10.1038/s41467-020-20230-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
167974pSEVA221T7RNAPExpresses T7RNAP in E. coli
167975pSEVA221MBPc-AID-T7RNAPExpresses fusion protein MBPc-AID-T7RNAP in E. coli
167976pSEVA221TrxA-AID-T7RNAPExpresses fusion protein TrxA-AID-T7RNAP in E. coli
167977pSEVA221AID-T7RNAPExpresses fusion protein AID-T7RNAP in E. coli
167978pSEVA221pmCDA1-T7RNAPExpresses fusion protein pmCDA1-T7RNAP in E. coli
167979pSEVA221rAPOBEC1-T7RNAPExpresses fusion protein rAPOBEC1-T7RNAP in E. coli
167980pSEVA221TadA*-T7RNAPExpresses fusion protein TadA*-T7RNAP in E. coli

Antibodies from Article