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XE239 Fz1-ER
(Plasmid #16816)

Full plasmid sequence is not available for this item.



This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16816 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Entrez Gene

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a fusion protein consisting of a FKBP domain at the amino terminus of rat Frizzled 1.
The expression of the protein is under the control of a CMV promoter. Once expressed the fusion protein enters the endoplasmic reticulum, where it remains trapped (and acts as a dominant negative Frizzled by trapping endogenous Frizzleds) unless one adds proprietary Ariad small molecule reagents that bind to and unfold the FKBP domain, allowing proteolysis and then processing and membrane targeting of the Frizzled 1. Note that the Frizzleds interact in a homo and heteromeric manner.

Notes for Use: Construct is 8251bp, made 9/16/02 by Ajamete, and
also called Fz1-ER/AKA pC4FZ1agg.

Reference: A. Kaykas et al., 2004, Nature Cell Biology vol. 6, pp. 52-58.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE239 Fz1-ER was a gift from Randall Moon (Addgene plasmid # 16816 ; ; RRID:Addgene_16816)
  • For your References section:

    Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type Frizzled in the endoplasmic reticulum by oligomerization. Kaykas A, Yang-Snyder J, Héroux M, Shah KV, Bouvier M, Moon RT. Nat Cell Biol. 2004 Jan . 6(1):52-8. 10.1038/ncb1081 PubMed 14688793