pBS drosophila armadillo
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16829||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS (+)
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. melanogaster (fly)
Insert Size (bp)2700
Entrez Genearm (a.k.a. Dmel_CG11579, ARM, Arm, CG11579, Dm Arm, Dmel\CG11579, EG:86E4.6, beta-Cat, beta-cat, beta-cat-arm, beta-catenin, beta-catenin/Arm, betacat, bgr;-Cat, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Description: Full coding sequence of Drosophila armadillo in pBluescript (+)KS'.
T7 primer is at the 5' end, followed by BamHI site at nt 150 of arm cDNA, and the initiation ATG is 6-8nt downstream. There is an EcoRI site at nt 287 of the arm cDNA, and a stop at nt 2704, followed by an Eco RI site at nt 2770 and a Bam HI at 2820. Thus the insert can best be obtained with a BamHI digest.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS drosophila armadillo was a gift from Eric Wieschaus (Addgene plasmid # 16829 ; http://n2t.net/addgene:16829 ; RRID:Addgene_16829)
For your References section:The segment polarity gene armadillo encodes a functionally modular protein that is the Drosophila homolog of human plakoglobin. Peifer M, Wieschaus E. Cell. 1990 Dec 21. 63(6):1167-76. 10.1016/0092-8674(90)90413-9 PubMed 2261639