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pBS drosophila armadillo
(Plasmid #16829)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16829 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS (+)
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    armadillo
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2700
  • Entrez Gene
    arm (a.k.a. Dmel_CG11579, ARM, Arm, CG11579, Dm Arm, Dmel\CG11579, EG:86E4.6, beta-Cat, beta-cat, beta-cat-arm, beta-catenin, beta-catenin/Arm, betacat, bgr;-Cat, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Full coding sequence of Drosophila armadillo in pBluescript (+)KS'.

T7 primer is at the 5' end, followed by BamHI site at nt 150 of arm cDNA, and the initiation ATG is 6-8nt downstream. There is an EcoRI site at nt 287 of the arm cDNA, and a stop at nt 2704, followed by an Eco RI site at nt 2770 and a Bam HI at 2820. Thus the insert can best be obtained with a BamHI digest.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS drosophila armadillo was a gift from Eric Wieschaus (Addgene plasmid # 16829 ; http://n2t.net/addgene:16829 ; RRID:Addgene_16829)
  • For your References section:

    The segment polarity gene armadillo encodes a functionally modular protein that is the Drosophila homolog of human plakoglobin. Peifer M, Wieschaus E. Cell. 1990 Dec 21. 63(6):1167-76. 10.1016/0092-8674(90)90413-9 PubMed 2261639