pRD443
(Plasmid
#168469)
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PurposeFor the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168469 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4798
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNLS-mScarlet-I-H-NSdbd-NLS
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Alt namemScarlet-I HI-NESS
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SpeciesSynthetic
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Insert Size (bp)921
- Promoter CMV promoter
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The pcDNA5/FRT/TO backbone has been modified by the addition of a Puromycin resistance gene (Luijsterburg lab, LUMC, Leiden, The Netherlands).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRD443 was a gift from Remus Dame (Addgene plasmid # 168469 ; http://n2t.net/addgene:168469 ; RRID:Addgene_168469) -
For your References section:
HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein. Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT. Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993. 10.1093/nar/gkab993 PubMed 34734265
