HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein.
Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT
Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993. PubMed Article
Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT
Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 163097 | pRD128 | Expression of mTurquoise2_H-NSdbd in Escherichia coli (and, potentially, other bacteria) |
| 163098 | pRD129 | Expression of mEos3.2_H-NSdbd in Escherichia coli (and, potentially, other bacteria) |
| 163099 | pRD188 | Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA |
| 163100 | pRD190 | Expression of mEos3.2 in eukaryotes to stain the nuclear compartment |
| 163101 | pRD198 | Expression of mEGFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria) |
| 163102 | pRD395 | Expression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria) |
| 163103 | pRD396 | Expression of eYFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria) |
| 163104 | pRD397 | Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA |
| 163105 | pRD398 | Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA |
| 163106 | pRD399 | Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA |
| 163107 | pRD400 | Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA |
| 163108 | pRD403 | Expression of mScarlet-I_H-NSdbd in Escherichia coli (and, potentially, other bacteria) |
| 163109 | pRD404 | Expression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria) |
| 163110 | pRD405 | Expression of mScarlet-I_H-NSdbd in eukaryotes for visualisation of nuclear DNA |
| 163111 | pRD406 | Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA |
| 163112 | pRD421 | Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS |
| 168463 | pRD437 | For the insertion pf NLS-mEos3.2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system |
| 168464 | pRD438 | For the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system |
| 168465 | pRD439 | For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system |
| 168466 | pRD440 | For the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system |
| 168467 | pRD441 | For the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system |
| 168468 | pRD442 | For the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system |
| 168469 | pRD443 | For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system |
| 168470 | pRD444 | For the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system |
| 168774 | pRD393 | Expression of mEos3.2_H-NSdbd in Mycobacterium |
| 168775 | pRD445 | Expression of mEos3.2 in Mycobacterium |