pCIG2-Flag-mNuak1 WT
(Plasmid
#168507)
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PurposeFor expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)
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Depositing Lab
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Depositing OrganizationScripps California (The Scripps Research Institute)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 168507-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 168507-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCIG2
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Vector typeMammalian Expression
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Selectable markersIRES-EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNuak1
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SpeciesM. musculus (mouse)
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Entrez GeneNuak1 (a.k.a. Omphk1, AU014801, AW494241, MGC90816, B230104P22Rik)
- Promoter CAG
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 168507-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 168507-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIG2-Flag-mNuak1 WT was a gift from Franck Polleux (Addgene plasmid # 168507 ; http://n2t.net/addgene:168507 ; RRID:Addgene_168507) -
For your References section:
Terminal axon branching is regulated by the LKB1-NUAK1 kinase pathway via presynaptic mitochondrial capture. Courchet J, Lewis TL Jr, Lee S, Courchet V, Liou DY, Aizawa S, Polleux F. Cell. 2013 Jun 20;153(7):1510-25. doi: 10.1016/j.cell.2013.05.021. 10.1016/j.cell.2013.05.021 PubMed 23791179
