PurposeExpression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||168513||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Entrez GeneLamp1 (a.k.a. LGP120)
- Promoter CAG
/ Fusion Protein
- mEmerald (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG mEmerald-LAMP1 was a gift from Franck Polleux (Addgene plasmid # 168513 ; http://n2t.net/addgene:168513 ; RRID:Addgene_168513)
For your References section:Terminal axon branching is regulated by the LKB1-NUAK1 kinase pathway via presynaptic mitochondrial capture. Courchet J, Lewis TL Jr, Lee S, Courchet V, Liou DY, Aizawa S, Polleux F. Cell. 2013 Jun 20;153(7):1510-25. doi: 10.1016/j.cell.2013.05.021. 10.1016/j.cell.2013.05.021 PubMed 23791179