XE172 antisense mouse Wnt-1-myc-pCDbeta-G
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16852||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeXenopus Expression
Growth in Bacteria
Gene/Insert nameantisense Wnt-1
SpeciesM. musculus (mouse)
Entrez GeneWnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
/ Fusion Protein
- internal myc tag
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer SV40pro-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Description: Antisense orientation of full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, cloned into the SV40 expression vector pCD-PS, but providing Xenopus beta globin 5' and 3' UTRs.
Species of Sequence Origin: mouse.
Notes for Use: Uncharacterized.
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished construct was generated by Julia Yang-Snyder.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE172 antisense mouse Wnt-1-myc-pCDbeta-G was a gift from Randall Moon (Addgene plasmid # 16852 ; http://n2t.net/addgene:16852 ; RRID:Addgene_16852)