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XE170 mouse Wnt-1-myc-pcDNA3
(Plasmid #16854)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16854 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression ; Xenopus Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Wnt1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Wnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
  • Tag / Fusion Protein
    • myc (internal)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, in the Stratagene vector, pcDNA3. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.

Species of Sequence Origin: mouse.

Notes for Use: Uncharacterized.

Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished version was generated by Julia Yang-Snyder.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE170 mouse Wnt-1-myc-pcDNA3 was a gift from Randall Moon (Addgene plasmid # 16854 ; http://n2t.net/addgene:16854 ; RRID:Addgene_16854)