XE170 mouse Wnt-1-myc-pcDNA3
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16854||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression ; Xenopus Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesM. musculus (mouse)
Entrez GeneWnt1 (a.k.a. Int, Int-1, Wnt-, Wnt-1, sw, swaying)
/ Fusion Protein
- myc (internal)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope, in the Stratagene vector, pcDNA3. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10.
Species of Sequence Origin: mouse.
Notes for Use: Uncharacterized.
Reference: The insert was published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989). This unpublished version was generated by Julia Yang-Snyder.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XE170 mouse Wnt-1-myc-pcDNA3 was a gift from Randall Moon (Addgene plasmid # 16854 ; http://n2t.net/addgene:16854 ; RRID:Addgene_16854)