ZP61 Isthmin
(Plasmid #16881)

• Depositing Lab: Randall Moon
• Publication: Zebrafish Probe Plasmids (unpublished)

Backbone

• Vector backbone: pSPORT1
• Backbone size w/o insert (bp): 4100
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: isthmin
• Species: D. rerio (zebrafish)
• Entrez Gene: ism1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Zebrafish Isthmin cDNA, clone 27j15 from the microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library) in pSPORT1. A clone that is slightly longer at the 5' end was identified in the 2nd array round, so this is not exactly full-length. For antisense probe synthesis.
Expressed on dorsal side early, in a dorsal high to ventral low gradient in margin, during gastrulation around axial mesoderm, later strongly at MHB. For antisense: Asp718, SP6.

Citation: G. Weidinger and R. T. Moon (2005). Development.