|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16908||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK-
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)1200
Entrez Geneher5 (a.k.a. her-5, zgc:136537)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
For probe, digest with XhoI, transcribe with T3 polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP34 Her5 was a gift from Randall Moon (Addgene plasmid # 16908 ; http://n2t.net/addgene:16908 ; RRID:Addgene_16908)
Map uploaded by the depositor.