ZP42 Vent-CS (ZV86)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16900||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)700
Entrez Genevent (a.k.a. vega2, vent1, wu:fe36b11)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Zvox-1 in CS2+. EcoRI-HpaI fragment from Zvox-1-2 was inserted into KS EcoRI-Sma as ZV80. EcoRI-XbaI fragment from this was inserted into CS2+ EcoRI-XbaI.
To make RNA: cut with Asp718, transcribe with SP6.
Constructed by: David Kimelman, 2/1/97.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP42 Vent-CS (ZV86) was a gift from Randall Moon (Addgene plasmid # 16900 ; http://n2t.net/addgene:16900 ; RRID:Addgene_16900)
Map uploaded by the depositor.