|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16882||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Entrez Geneaxin2 (a.k.a. axil, wu:fb37c08, zgc:111942)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Zebrafish axin2 cDNA (unclear if full-length or not) in pSPORT1. Clone 24h4 from microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library). For antisense probe synthesis.
Pretty ubiquitously expressed during gastrulation. Positively regulated by Wnt signaling.
For antisense: Asp718, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP60 Axin2 was a gift from Randall Moon (Addgene plasmid # 16882 ; http://n2t.net/addgene:16882 ; RRID:Addgene_16882)