Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11300||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS(-)
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)649
Entrez Generag1 (a.k.a. RAG-1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This is a rag1 PCR product. To make probes: antisense HindIII - T7, sense XbaI - T3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:rag1 short was a gift from Lisa Steiner (Addgene plasmid # 11300 ; http://n2t.net/addgene:11300 ; RRID:Addgene_11300)
For your References section:Characterization and expression of the recombination activating genes (rag1 and rag2) of zebrafish. Willett CE, Cherry JJ, Steiner LA. Immunogenetics. 1997 . 45(6):394-404. 10.1007/s002510050221 PubMed 9089097
Map uploaded by the depositor.