IgM (exon 1-3)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11301||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3948
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)828
Entrez Geneighm (a.k.a. ighm1, wu:fj14a08, zgc:101769)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer T7 (Common Sequencing Primers)
Clone A1 (exon 1-3) was amplified using primers zfCH1 (5'-TCAACCATCTGCGCCCCAGT) and cm3A (5'-TCCTGAGGCTTTGCACTT).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:IgM (exon 1-3) was a gift from Lisa Steiner (Addgene plasmid # 11301 ; http://n2t.net/addgene:11301 ; RRID:Addgene_11301)
For your References section:The immunoglobulin heavy-chain locus in zebrafish: identification and expression of a previously unknown isotype, immunoglobulin Z. Danilova N, Bussmann J, Jekosch K, Steiner LA. Nat Immunol. 2005 Mar . 6(3):295-302. 10.1038/ni1166 PubMed 15685175
Map uploaded by the depositor.