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IgM (exon 1-3)
(Plasmid #11301)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11301 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCRII
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IgM
  • Alt name
    mu
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    828
  • GenBank ID
    AY643753
  • Entrez Gene
    ighm (a.k.a. ighm1, wu:fj14a08, zgc:101769)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Clone A1 (exon 1-3) was amplified using primers zfCH1 (5'-TCAACCATCTGCGCCCCAGT) and cm3A (5'-TCCTGAGGCTTTGCACTT).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IgM (exon 1-3) was a gift from Lisa Steiner (Addgene plasmid # 11301 ; http://n2t.net/addgene:11301 ; RRID:Addgene_11301)
  • For your References section:

    The immunoglobulin heavy-chain locus in zebrafish: identification and expression of a previously unknown isotype, immunoglobulin Z. Danilova N, Bussmann J, Jekosch K, Steiner LA. Nat Immunol. 2005 Mar . 6(3):295-302. 10.1038/ni1166 PubMed 15685175