Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11260||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS-
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)1297
Entrez Genefoxn4 (a.k.a. whn, whna)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
To make probes: antisense XbaI - T3, sense XhoI - T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:foxn4 was a gift from Lisa Steiner (Addgene plasmid # 11260 ; http://n2t.net/addgene:11260 ; RRID:Addgene_11260)
For your References section:Expression of the winged helix/forkhead gene, foxn4, during zebrafish development. Danilova N, Visel A, Willett CE, Steiner LA. Brain Res Dev Brain Res. 2004 Oct 15. 153(1):115-9. 10.1016/j.devbrainres.2004.05.014 PubMed 15464224