pYFAC-pyroA
(Plasmid
#168976)
-
Purpose(Empty Backbone) Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; pyroA
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 168976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepYFAC-pyroA
- Backbone size (bp) 14581
-
Vector typeSynthetic Biology ; Expression in Aspergillus nidulans and related filamentous fungi
-
Selectable markersURA3 ; Af pyroA
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pYFAC-pyroA was a gift from Yit Heng Chooi (Addgene plasmid # 168976 ; http://n2t.net/addgene:168976 ; RRID:Addgene_168976) -
For your References section:
Heterologous biosynthesis of elsinochrome A sheds light on the formation of the photosensitive perylenequinone system. Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, Karton A, Piggott AM, Chooi YH. Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. eCollection 2019 Feb 7. 10.1039/c8sc02870b PubMed 30809363
Map uploaded by the depositor.
