pYFAC
(Plasmid #168982)

• Purpose: (Empty Backbone) Filamentous fungi vector. Also referred to as pYFAC-pyrG.
• Depositing Lab: Yit Heng Chooi
• Publication: Chooi et al Environ Microbiol. 2017 May;19(5):1975-1986. doi: 10.1111/1462-2920.13711. Epub 2017 Apr 10.

Backbone

• Vector backbone: pKW20088
• Backbone manufacturer: doi:10.1038/nchembio.1366
• Backbone size (bp): 14245
• Vector type: Synthetic Biology ; Heterologous expression in filamentous fungi.
• Selectable markers: URA3 ; Af pyrG

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For improved transformation efficiency, using a DH10B E coli such as 10-beta, and electroporation.
• Copy number: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5