ZP44 Wnt8 5' orf2
(Plasmid
#16898)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBluescript SK+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namewnt8 5'orf2
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Alt namewnt8
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)588
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Entrez Genewnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
588 bp PCR product ligated into BamHI and EcoRI cut pSK+.
For probe: cut with BamHI, use T7 RNA polymerase.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZP44 Wnt8 5' orf2 was a gift from Randall Moon (Addgene plasmid # 16898 ; http://n2t.net/addgene:16898 ; RRID:Addgene_16898)