ZP44 Wnt8 5' orf2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16898||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK+
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namewnt8 5'orf2
SpeciesD. rerio (zebrafish)
Insert Size (bp)588
Entrez Genewnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
588 bp PCR product ligated into BamHI and EcoRI cut pSK+.
For probe: cut with BamHI, use T7 RNA polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP44 Wnt8 5' orf2 was a gift from Randall Moon (Addgene plasmid # 16898 ; http://n2t.net/addgene:16898 ; RRID:Addgene_16898)