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ZP43 Wnt8 3' orf1
(Plasmid #16899)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16899 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-T easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wnt8 3' orf1
  • Alt name
    wnt8
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    530
  • Entrez Gene
    wnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

530 bp PCR product ligated into vector pGEMTeasy. Purpose of clone is to provide an in situ probe specific for the first open reading frame of the bicistronic wnt8 RNA.

To make antisense probe: cut with Sph1, transcribe with SP6.

Citation: A. Lekven et al., 2001, Developmental Cell.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP43 Wnt8 3' orf1 was a gift from Randall Moon (Addgene plasmid # 16899 ; http://n2t.net/addgene:16899 ; RRID:Addgene_16899)