ZP43 Wnt8 3' orf1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16899||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGEM-T easy
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namewnt8 3' orf1
SpeciesD. rerio (zebrafish)
Insert Size (bp)530
Entrez Genewnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
530 bp PCR product ligated into vector pGEMTeasy. Purpose of clone is to provide an in situ probe specific for the first open reading frame of the bicistronic wnt8 RNA.
To make antisense probe: cut with Sph1, transcribe with SP6.
Citation: A. Lekven et al., 2001, Developmental Cell.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZP43 Wnt8 3' orf1 was a gift from Randall Moon (Addgene plasmid # 16899 ; http://n2t.net/addgene:16899 ; RRID:Addgene_16899)
Map uploaded by the depositor.