hp-50-0-50%GC-Right-half-120bp
(Plasmid
#169207)
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Purpose50-0-50%GC-Right-half-120bp-hairpin
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCR-XL-TOPO
- Backbone size w/o insert (bp) 3519
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name50-0-50%GC-Right-half-120bp-hairpin
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SpeciesSynthetic
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Insert Size (bp)1700
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer GGT AAC GCC AGG GTT TTC CCA GTC AC
- 3′ sequencing primer GGA TAA CAA TTT CAC ACA GGA AAC AGC TAT GAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Since the 120nt hairpin cannot be cloned in it's entirety the two halves (Left & Right) are provided here.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hp-50-0-50%GC-Right-half-120bp was a gift from Tom Perkins (Addgene plasmid # 169207 ; http://n2t.net/addgene:169207 ; RRID:Addgene_169207) -
For your References section:
Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay. Taylor AMK, Okoniewski SR, Uyetake L, Perkins TT. Methods Mol Biol. 2022;2478:273-312. doi: 10.1007/978-1-0716-2229-2_10. 10.1007/978-1-0716-2229-2_10 PubMed 36063324
