Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay.
Taylor AMK, Okoniewski SR, Uyetake L, Perkins TT
Methods Mol Biol. 2022;2478:273-312. doi: 10.1007/978-1-0716-2229-2_10. PubMed Article
Taylor AMK, Okoniewski SR, Uyetake L, Perkins TT
Methods Mol Biol. 2022;2478:273-312. doi: 10.1007/978-1-0716-2229-2_10. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169196 | hp-50%GC-20bp | Force-activating hairpin for 3'-to-5' helicase |
| 169197 | hp-50%GC-40bp | Force-activating hairpin for 3'-to-5' helicase |
| 169198 | hp-50%GC-40bp-Revbindsite | Force-activating hairpin for 5'-to-3' helicase |
| 169199 | hp-66%GC-20bp | Force-activating hairpin for 3'-to-5' helicase |
| 169200 | hp-66%GC-40bp | Force-activating hairpin for 3'-to-5' helicase |
| 169201 | hp-75%GC-20bp | Force-activating hairpin for 3'-to-5' helicase |
| 169202 | hp-75%GC-40bp | Force-activating hairpin for 3'-to-5' helicase |
| 169203 | hp-100%GC-20bp | Force-activating hairpin for 3'-to-5' helicase |
| 169204 | hp-50%GC-Left-half-120bp | Force-activating hairpin for 3'-to-5' helicase |
| 169205 | hp-50%GC-Right-half-120bp | Force-activating hairpin for 3'-to-5' helicase |
| 169206 | hp-50-0-50%GC-Left-half-120bp | Force-activating hairpin for 3'-to-5' helicase |
| 169207 | hp-50-0-50%GC-Right-half-120bp | 50-0-50%GC-Right-half-120bp-hairpin |