Nsp3 Mac1 - P43 (Oxford)
(Plasmid
#169210)
-
PurposeExpression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 169210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepET22b(+)
- Backbone size w/o insert (bp) 5358
- Total vector size (bp) 5928
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSARS-CoV-2 Nsp3 Mac1
-
Alt nameNsp3 ADRP
-
SpeciesSARS-CoV-2
-
Insert Size (bp)570
-
GenBank IDNC_045512.2
- Promoter T7
-
Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 Fwd (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byGenscript
-
Terms and Licenses
-
Industry Terms
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Nsp3 Mac1 - P43 (Oxford) was a gift from James Fraser (Addgene plasmid # 169210)