Nsp3 Mac1 - P43 (Oxford)
(Plasmid #169210)

• Purpose: Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal
• Depositing Lab: James Fraser
• Publication: Fragment Binding to the Nsp3 Macrodomain of SARS-CoV-2 Identified Through Crystallographic Screening and Computational Docking (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	169210	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Pending

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pET22b(+)
  (Search Vector Database)
• Backbone size w/o insert (bp): 5358
• Total vector size (bp): 5928
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 Nsp3 Mac1
• Alt name: Nsp3 ADRP
• Species: SARS-CoV-2
• Insert Size (bp): 570
• GenBank ID: NC_045512.2
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Fwd

Resource Information

• Supplemental Documents:
  • sars-2-nsp3-mac1-oxford.gb
• A portion of this plasmid was derived from a plasmid made by: Genscript
• Terms and Licenses:
  • UBMTA
• Industry Terms:
  • Not Available to Industry