Nsp3 Mac1 - P43 (Oxford)
(Plasmid #169210)

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal
Depositing Lab

James Fraser
Publication

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/eabf8711. doi: 10.1126/sciadv.abf8711. Print 2021 Apr. ( How to cite )
Sequence Information
- Sequences (1)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	169210	Standard format: Plasmid sent in bacteria as agar stab	1	$75 *	Pending

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Backbone

Vector backbone
pET22b(+)
(Search Vector Database)
Backbone size w/o insert (bp) 5358
Total vector size (bp) 5928
Vector type
Bacterial Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
SARS-CoV-2 Nsp3 Mac1
Alt name
Nsp3 ADRP
Species
SARS-CoV-2
Insert Size (bp)
570
GenBank ID
NC_045512.2
Promoter T7
Tag / Fusion Protein
- 6xHis (N terminal on insert)

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site NdeI (not destroyed)
3′ cloning site XhoI (not destroyed)
5′ sequencing primer T7 Fwd

(Common Sequencing Primers)

Resource Information

Supplemental Documents
- sars-2-nsp3-mac1-oxford.gb
A portion of this plasmid was derived from a plasmid made by
Genscript
Terms and Licenses
- UBMTA
Industry Terms
- Industry MTA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

Nsp3 Mac1 - P43 (Oxford) was a gift from James Fraser (Addgene plasmid # 169210)
For your References section:

Fragment binding to the Nsp3 macrodomain of SARS-CoV-2 identified through crystallographic screening and computational docking. Schuller M, Correy GJ, Gahbauer S, Fearon D, Wu T, Diaz RE, Young ID, Carvalho Martins L, Smith DH, Schulze-Gahmen U, Owens TW, Deshpande I, Merz GE, Thwin AC, Biel JT, Peters JK, Moritz M, Herrera N, Kratochvil HT, Aimon A, Bennett JM, Brandao Neto J, Cohen AE, Dias A, Douangamath A, Dunnett L, Fedorov O, Ferla MP, Fuchs MR, Gorrie-Stone TJ, Holton JM, Johnson MG, Krojer T, Meigs G, Powell AJ, Rack JGM, Rangel VL, Russi S, Skyner RE, Smith CA, Soares AS, Wierman JL, Zhu K, O'Brien P, Jura N, Ashworth A, Irwin JJ, Thompson MC, Gestwicki JE, von Delft F, Shoichet BK, Fraser JS, Ahel I. Sci Adv. 2021 Apr 14;7(16). pii: 7/16/eabf8711. doi: 10.1126/sciadv.abf8711. Print 2021 Apr. 10.1126/sciadv.abf8711 PubMed 33853786

Nsp3 Mac1 - P43 (Oxford) (Plasmid #169210)