eGFP-CBP
(Plasmid #169211)

• Purpose: Expression of eGFP-CBP protein for chitin visualization
• Depositing Lab: James Fraser
• Publication: Type 2 innate immunity regulates hair follicle homeostasis necessary to control Demodex pathosymbionts. (unpublished)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	169211	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Pending

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pGV358
  (Search Vector Database)
• Backbone size w/o insert (bp): 5262
• Total vector size (bp): 6816
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Chitinase A1 Chitin Binding Domain
• Alt name: ChiA1 CBD
• Species: Bacillus circulans
• Insert Size (bp): 1554
• Promoter: T7
• Tags / Fusion Proteins:
  • eGFP (N terminal on insert)
  • Mxe Gyr Intein (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Sy Redding, University of California San Francisco
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry