eGFP-CBP
(Plasmid
#169211)
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PurposeExpression of eGFP-CBP protein for chitin visualization
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Depositing Lab
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 169211 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGV358
- Backbone size w/o insert (bp) 5262
- Total vector size (bp) 6816
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChitinase A1 Chitin Binding Domain
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Alt nameChiA1 CBD
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SpeciesBacillus circulans
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Insert Size (bp)1554
- Promoter T7
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Tags
/ Fusion Proteins
- eGFP (N terminal on insert)
- Mxe Gyr Intein (N terminal on insert)
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySy Redding, University of California San Francisco
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Terms and Licenses
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Industry Terms
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
eGFP-CBP was a gift from James Fraser (Addgene plasmid # 169211)