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(Plasmid #134898)


Item Catalog # Description Quantity Price (USD)
Plasmid 134898 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5711
  • Total vector size (bp) 6440
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    Proliferating Cell Nuclear Antigen
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    fully synthetic, codon optimized for bacterial expression
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter T7
  • Tag / Fusion Protein
    • untagged

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a codon optimized untagged PCNA cDNA expression vector. N- or C-terminal tags on PCNA inhibit its trimerization, so purification is instead via several steps of anion exchange and size exclusion chromatography. Protein purification protocol is as in Tan et al. Plos One 2020

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET16b-PCNA was a gift from Andrew Deans (Addgene plasmid # 134898 ; ; RRID:Addgene_134898)
  • For your References section:

    Preparation and purification of mono-ubiquitinated proteins using Avi-tagged ubiquitin. Tan W, Murphy VJ, Charron A, van Twest S, Sharp M, Constantinou A, Parker MW, Crismani W, Bythell-Douglas R, Deans AJ. PLoS One. 2020 Feb 24;15(2):e0229000. doi: 10.1371/journal.pone.0229000. eCollection 2020. 10.1371/journal.pone.0229000 PubMed 32092106