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p36-p37-p38-p40-pET-Duet-1
(Plasmid #175049)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 175049 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 9849
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Replication factor C subunits 2-5
  • Alt name
    Uniprot P35250, P40938, P35249, P40937
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • Entrez Gene
    RFC4 (a.k.a. A1, RFC37)
  • Entrez Gene
    RFC2 (a.k.a. RFC40)
  • Entrez Gene
    RFC3 (a.k.a. RFC38)
  • Entrez Gene
    RFC5 (a.k.a. RFC36)
  • Promoter T7 promoter

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cloned by Kadyrov et al. 10.1073/pnas.0903654106 Plasmids graciously provided by Drs M. Hedglin and Dr. S. J. Benkovic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p36-p37-p38-p40-pET-Duet-1 was a gift from Brian Kelch (Addgene plasmid # 175049 ; http://n2t.net/addgene:175049 ; RRID:Addgene_175049)
  • For your References section:

    Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch. Gaubitz C, Liu X, Magrino J, Stone NP, Landeck J, Hedglin M, Kelch BA. Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23571-23580. doi: 10.1073/pnas.2007437117. Epub 2020 Sep 9. 10.1073/pnas.2007437117 PubMed 32907938