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Structure of the human clamp loader reveals an autoinhibited conformation of a substrate-bound AAA+ switch.
Gaubitz C, Liu X, Magrino J, Stone NP, Landeck J, Hedglin M, Kelch BA
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23571-23580. doi: 10.1073/pnas.2007437117. Epub 2020 Sep 9. PubMed Article

Plasmids from Article

ID Plasmid Purpose
175043pCDF-1b-RFC140ΔN555Overexpression of human (Rfc1) p140ΔN555 in E.coli
175049p36-p37-p38-p40-pET-Duet-1Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli
175050p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoAOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli
175051p36-p37-p38-p40-pET-Duet-1-Rfc3-T76DOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Antibodies from Article