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p11d-tRPA-32Asp8
(Plasmid #102617)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102617 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RPA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPA1 (a.k.a. HSSB, MST075, REPA1, RF-A, RP-A, RPA70)

Gene/Insert 2

  • Gene/Insert name
    RPA3
  • Entrez Gene
    RPA3 (a.k.a. REPA3, RP-A p14)

Gene/Insert 3

  • Gene/Insert name
    RPA2
  • Mutation
    with aspartic acid substituted phosphorylation domain
  • Entrez Gene
    RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Note: The RPA2 gene in this plasmid is a variant that also contains a T88I mutation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p11d-tRPA-32Asp8 was a gift from Marc Wold (Addgene plasmid # 102617 ; http://n2t.net/addgene:102617 ; RRID:Addgene_102617)
  • For your References section:

    The phosphorylation domain of the 32-kDa subunit of replication protein A (RPA) modulates RPA-DNA interactions. Evidence for an intersubunit interaction. Binz SK, Lao Y, Lowry DF, Wold MS. J Biol Chem. 2003 Sep 12;278(37):35584-91. Epub 2003 Jun 20. 10.1074/jbc.M305388200 PubMed 12819197