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Addgene

p11d-tRPA-32Ala9
(Plasmid #102616)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 102616 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET11d
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RPA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    RPA1 (a.k.a. HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RPA3
  • Entrez Gene
    RPA3 (a.k.a. REPA3, RP-A p14)

Gene/Insert 3

  • Gene/Insert name
    RPA2
  • Mutation
    with an alanine substituted phosphorylation domain
  • Entrez Gene
    RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

nt regions for each gene:
RPA70 5317-7164; RPA32 8413-9226; RPA14 7706-8068

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p11d-tRPA-32Ala9 was a gift from Marc Wold (Addgene plasmid # 102616 ; http://n2t.net/addgene:102616 ; RRID:Addgene_102616)