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pET3d-SF-DCTN1-TS
(Plasmid #169375)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 169375 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pet3d
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 8500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dynactin subunit 1 isoform 1
  • Alt name
    DCTN1
  • Alt name
    p150 glued
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3900
  • Mutation
    Point mutations: A139T, I304T, Q322R, T516A, E577G, A590G, E1011G
  • GenBank ID
    NM_004082.5 NM_004082.5
  • Entrez Gene
    DCTN1 (a.k.a. DAP-150, DP-150, P135)
  • Tags / Fusion Proteins
    • SplitFlAsH Tag (N terminal on insert)
    • TwinStrep Tag (C terminal on insert)
    • 6xHis Tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer T7 promotor fwd primer
  • 3′ sequencing primer T7 terminator rev primer
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The DCTN1 gene was amplified from the pENTR221-DCTN1 vector that was ordered from the DKFZ Vector and Clone Repository.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3d-SF-DCTN1-TS was a gift from Bernd Bukau (Addgene plasmid # 169375 ; http://n2t.net/addgene:169375 ; RRID:Addgene_169375)
  • For your References section:

    Interactions between nascent proteins translated by adjacent ribosomes drive homomer assembly. Bertolini M, Fenzl K, Kats I, Wruck F, Tippmann F, Schmitt J, Auburger JJ, Tans S, Bukau B, Kramer G. Science. 2021 Jan 1;371(6524):57-64. doi: 10.1126/science.abc7151. 10.1126/science.abc7151 PubMed 33384371