pET3d-SF-DCTN1-TS
(Plasmid #169375)

• Purpose: E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
• Depositing Lab: Bernd Bukau
• Publication: Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi: 10.1126/science.abc7151.

Backbone

• Vector backbone: pet3d
• Backbone size w/o insert (bp): 4600
• Total vector size (bp): 8500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: dynactin subunit 1 isoform 1
• Alt name: DCTN1
• Alt name: p150 glued
• Species: H. sapiens (human)
• Insert Size (bp): 3900
• Mutation: Point mutations: A139T, I304T, Q322R, T516A, E577G, A590G, E1011G
• GenBank ID: NM_004082.5 NM_004082.5
• Entrez Gene: DCTN1 (a.k.a. DAP-150, DP-150, P135)
• Tags / Fusion Proteins:
  • SplitFlAsH Tag (N terminal on insert)
  • TwinStrep Tag (C terminal on insert)
  • 6xHis Tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 promotor fwd primer
• 3′ sequencing primer: T7 terminator rev primer

Resource Information

• Supplemental Documents:
  • pET3d-SF-DCTN1-TS.gb
• A portion of this plasmid was derived from a plasmid made by: The DCTN1 gene was amplified from the pENTR221-DCTN1 vector that was ordered from the DKFZ Vector and Clone Repository.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry