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Interactions between nascent proteins translated by adjacent ribosomes drive homomer assembly.
Bertolini M, Fenzl K, Kats I, Wruck F, Tippmann F, Schmitt J, Auburger JJ, Tans S, Bukau B, Kramer G
Science. 2021 Jan 1;371(6524):57-64. doi: 10.1126/science.abc7151. PubMed Article

Plasmids from Article

ID Plasmid Purpose
169374pET3a-SF-LMNC-TSE. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
169375pET3d-SF-DCTN1-TSE. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

Antibodies from Article