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pET3a-SF-LMNC-TS
(Plasmid #169374)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 169374 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET3a
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 6365
  • Modifications to backbone
    NdeI + BamHI were used to open the MCS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LMNA residues 31-542
  • Alt name
    gene: LMNA
  • Alt name
    Protein isoform: Lamin C
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1752
  • Mutation
    deleted amino acid 1-30 (unstructured head domain), two silent mutations in the LMNA gene (isolated from U2OS cells) at plasmid position C4960T, G5157A
  • GenBank ID
    NM_001282625
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
  • Promoter Native pET3a promotor
  • Tags / Fusion Proteins
    • Split-FlAsH Tag (N terminal on insert)
    • TwinStrep Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promotor fwd primer
  • 3′ sequencing primer T7 terminator rev primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3a-SF-LMNC-TS was a gift from Bernd Bukau (Addgene plasmid # 169374 ; http://n2t.net/addgene:169374 ; RRID:Addgene_169374)
  • For your References section:

    Interactions between nascent proteins translated by adjacent ribosomes drive homomer assembly. Bertolini M, Fenzl K, Kats I, Wruck F, Tippmann F, Schmitt J, Auburger JJ, Tans S, Bukau B, Kramer G. Science. 2021 Jan 1;371(6524):57-64. doi: 10.1126/science.abc7151. 10.1126/science.abc7151 PubMed 33384371