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(Plasmid #197930)


Item Catalog # Description Quantity Price (USD)
Plasmid 197930 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 6266
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    7-HCou Aminoacyl tRNA synthetase
  • Insert Size (bp)
  • Promoter glnS' and lambda(ts)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tRNA opt
  • Insert Size (bp)
  • Promoter proK

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUP-(2xCouRS-tRNAopt) was a gift from Kenneth Johnson (Addgene plasmid # 197930 ; ; RRID:Addgene_197930)
  • For your References section:

    Optimized incorporation of an unnatural fluorescent amino acid affords measurement of conformational dynamics governing high-fidelity DNA replication. Dangerfield TL, Johnson KA. J Biol Chem. 2020 Dec 11;295(50):17265-17280. doi: 10.1074/jbc.RA120.015557. Epub 2020 Oct 5. 10.1074/jbc.RA120.015557 PubMed 33020184